IL3RA polyclonal antibody

Product Specifications

Background

IL3RA/CD123 is the alpha subunit of an IL-3 receptor that also contains the common beta chain (βc) shared with receptors for other cytokines. IL3RA/CD123 alone binds to IL-3 with high specificity but low affinity and it does not transduce intracellular signaling, while βc alone does not bind to IL-3 appreciably. Together, IL3RA/CD123 and βc form the functional high-affinity receptor for IL-3. IL3RA/CD123 is mainly expressed on the hematopoietic progenitor cells, primarily the granulocytic and monocytic linages . IL3RA/CD123 is highly expressed on leukemic stem cells in the majority of AML (acute myeloid leukemia) patients and is correlated with poor prognosis. In addition, overexpression of IL3RA/CD123 has also been reported for other hematopoietic malignancies. It is considered as a valuable therapeutic target for these malignancies.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

Interleukin-3 receptor subunit alpha; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA; CD_antigen: CD123

Swiss Prot

P26951

Reactivity

Human, Rat, Mouse

Immunogen

Synthetic peptide, corresponding to Human IL3RA.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:1000~1:2000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 60 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IL3RA polyclonal antibody detects endogenous levels of IL3RA protein.

Applications Notes

Western blot (WB) analysis of IL3RA polyclonal antibody at 1:1000 dilution Lane1:CT-26 whole cell lysate (30ug) Lane2:C6 whole cell lysate (30ug) Lane3:HepG2 whole cell lysate (30ug) Lane4:HCT116 whole cell lysate (30ug) Lane5:A549 whole cell lysate (30ug)