LAMP2a polyclonal antibody

Product Specifications

Background

Lysosome-associated membrane proteins (LAMP) are glycosylated type I membrane proteins that play a role in the biogenesis of the pigment melanin. LAMP-1 (also designated CD107A) and LAMP-2 (also designated CD107B) are involved in a variety of functions, including cellular adhesion, and are thought to participate in the process of tumor invasion and metastasis. Newly synthesized LAMP-1 and LAMP-2 proteins are sorted at the trans Golgi network and are transported intracellularly via a pathway that is distinct from the clathrin-coated vesicles used for the mannose-6 phosphate receptor. LAMP-1 is expressed on the surface of thrombin-activated but not resting platelets, and it is thought to be involved in the adhesive, prothrombic properties of these cells. Both LAMP-1 and LAMP-2 are involved in maintaining lysosome acidity and protecting the lysosomal membranes from autodigestion, and their expression is increased in patients with lysosomal storage disorders.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Lysosome-associated membrane glycoprotein 2; LAMP-2; Lysosome-associated membrane protein 2; CD107 antigen-like family member B; LGP-96; CD107b; LAMP2

Swiss Prot

P13473

Reactivity

Human, Rat, Mouse

Immunogen

Synthetic peptide, corresponding to Human LAMP2a.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 38 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

LAMP2a polyclonal antibody detects endogenous levels of LAMP2a protein.

Applications Notes

Western blot (WB) analysis of LAMP2a polyclonal antibody at 1:500 dilution LaneA:A375 cell membrane lysate (40ug) LaneB:DLD cell membrane lysate (40ug) LaneC:The Lung tissue lysate of Mouse (40ug) LaneD:The Liver tissue lysate of Mouse (40ug) LaneE:The Lung tissue lysate of Rat (40ug) LaneF:The Liver tissue lysate of Rat (40ug)