ULK1 polyclonal antibody

Product Specifications

Background

Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog (ATG1; Hatg1; Unc-51-like kinase 1; ULK1; KIAA0722

Swiss Prot

O75385

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to Human ULK1.

Conjugation

Unconjugated

Related Pathways

PI3K AKT signaling, MTOR signaling

Applications

WB IHC

Dilution

WB:1:500-1:1,000 IHC:1:50-1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 150 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ULK1 polyclonal antibody detects endogenous levels of ULK1 protein.

Applications Notes

Western blot (WB) analysis of ULK1 polyclonal antibody at 1:500 dilution LaneA:A549 whole cell lysate (40ug) LaneB:HEK293T whole cell lysate (40ug) LaneC:AML-12 whole cell lysate (40ug) LaneD:C6 whole cell lysate (40ug) LaneE:Panc1 whole cell lysate (40ug)