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CYP3A4 polyclonal antibody

Product Specifications

Background

CYP3A, the most abundant p450 enzyme in human liver, is responsible for the metabolism of more than 50% of the clinical test & lt; RTI ID = 0.0 & gt; (cYP3A) genes encode monooxygenases-enzymes which catalyze drug metabolism and the synthesis of cholesterol, steroids and other lipids. CYP3A family members localize in organs that associate with drug disposition, including the liver, gastrointestinal tract and kidney. The CYP3A cluster maps to gene locus 7q22.1 and consists of four genes (CYP3A4, CYP3A5, CYP3A7 and CYP3A43) and two pseudogenes (CYP3A4 is abundant in the endoplasmic reticulum of liver cells and upper intestinal enterocytes. CYP3A4 expression is isducible by glucocorticoids pharmacological agents.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

CYP3A4; CP33; CP34; CYP3A; CYP3A3; CYPIIIA3; CYPIIIA4; HLP; NF-25; P450C3; P450PCN1

Swiss Prot

P08684

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 244-503 of human CYP3A4 (NP_059488.2) .

Conjugation

Unconjugated

Applications

WB IF/ICC

Dilution

WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 57 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CYP3A4 polyclonal antibody detects endogenous levels of CYP3A4 protein.

Applications Notes

Immunofluorescence analysis of HeLa cells using CYP3A4 antibody at dilution of 1:100 (40x lens) .

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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