ATF3 (N159) polyclonal antibody

Product Specifications

Background

Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. It encodes a protein with a calculated molecular mass of 22 kD. ATF3 represses rather than activates transcription from promoters with ATF binding elements. An alternatively spliced form of ATF3 (ATF3 delta Zip) encodes a truncated form ATF3 protein lacking the leucine zipper protein-dimerization motif and does not bind to DNA. In contrast to ATF3, ATF3 delta Zip stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes. Three transcript variants encoding two different isoforms have been found for this gene.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Cyclic AMP-dependent transcription factor ATF-3; cAMP-dependent transcription factor ATF-3; Activating transcription factor 3; ATF3; ATF 3

Swiss Prot

P18847

Reactivity

Human, Rat

Immunogen

Synthetic peptide, corresponding to Human ATF3.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:1000~1:2000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 24 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ATF-3 (N159) polyclonal antibody detects endogenous levels of ATF-3 protein.

Applications Notes

Western blot (WB) analysis of ATF3 (N159) pAb at 1:500 dilution Lane1:L02 whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug)