ASCL1 (E158) polyclonal antibody
Product Specifications
Background
Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Involved in the regulation of neuroendocrine cell development in the glandular stomach (By similarity) .
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
Achaete-scute homolog 1; ASH-1; Hash1; Class A basic helix-loop-helix protein 46; bHLHa46
Swiss Prot
P50553
Reactivity
Rat, Mouse, Human
Immunogen
Synthetic peptide, corresponding to Human ASCL1.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:1000~1:2000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 35 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
ASCL1 (E158) polyclonal antibody detects endogenous levels of ASCL1 protein.
Applications Notes
Western blot (WB) analysis of ASCL1 (E158) polyclonal antibody at 1:1000 dilution Lane1:The brain tissue lysate of mouse (30ug) Lane2:The brain tissue lysate of Rat (30ug) Lane3:K562 whole cell lysate (30ug) Lane4:BV2 whole cell lysate (30ug) Lane5:A2780 cell membrane lysate (30ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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