NLK monoclonal antibody
Product Specifications
Background
Nemo-like kinase (NLK) is a serine/threonine-protein kinase that regulates multiple signaling pathways, including Wnt/β-catenin, TGFβ, IL-6, and Notch. NLK contributes to cell proliferation, differentiation, cell fate determination during early embryogenesis and nervous system development in vertebrates. Recent studies showed that NLK is aberrantly expressed in various types of cancer where it regulates cancer cell proliferation, migration, invasion and survival. NLK is localized predominantly in nucleus and at a lower level in cytoplasm. Homodimerization of NLK is required for its activation and nuclear localization. NLK is activated via intermolecular autophosphorylation at Thr286. NLK interacts with and phosphorylates a number of transcription factors including FOXO1, FOXO4, MYB, NOTCH1 and TCF7L2/TCF4, and LEF-1/TCF. NLK also associates with E3 ubiquitin ligase NARF and Raptor and regulates their function.
CAS Number
9007-83-4
Structure Composition
Mouse IgG2a. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Name Alternative
LAK1; Serine/threonine-protein kinase NLK; Nemo-like kinase; Protein LAK1
Swiss Prot
Q9UBE8
Reactivity
Human
Immunogen
Recombinant fusion protein of human NLK. The exact sequence is proprietary.
Conjugation
Unconjugated
Related Pathways
Wntβ catenin signaling
Applications
WB
Dilution
WB (1/500 - 1/1000)
Purity
This antibody is purified through a protein G column.
Modification
Unmodification
Molecular Weight
~ 70 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of NLK protein.
Applications Notes
Western blot analysis of NLK expression in SiHa (A), Hela (B) whole cell lysates.
Host or Source
Mouse
Available Sizes
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
