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CYLD monoclonal antibody

Product Specifications

Background

CYLD is a cytoplasmic deubiquitinating enzyme encoded by a tumor suppressor gene altered in individuals diagnosed with cylindromatosis, a genetic condition characterized by benign tumors of skin appendages. Functional CYLD deubiquitinase regulates inflammation and cell proliferation by down regulating NF-κB signaling through removal of ubiquitin chains from several NF-κB pathway proteins. CYLD is a negative regulator of proximal events in Wnt/β-catenin signaling and is a critical regulator of natural killer T cell development. The transcription factor Snail can inhibit CYLD expression, resulting in melanoma cell proliferation.

CAS Number

9007-83-4

Structure Composition

Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

CYLD1; KIAA0849; Ubiquitin carboxyl-terminal hydrolase CYLD; Deubiquitinating enzyme CYLD; Ubiquitin thioesterase CYLD; Ubiquitin-specific-processing protease CYLD

Swiss Prot

Q9NQC7

Reactivity

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CYLD. The exact sequence is proprietary.

Conjugation

Unconjugated

Related Pathways

Regulation of Apoptosis, Death Receptor Signaling, Inhibition of Apoptosis, NF-kB Signaling, Wntβ catenin signaling

Applications

WB, FC

Dilution

WB (1/500 - 1/2000), FC (1/10 - 1/50)

Purity

This antibody is purified through a protein G column.

Modification

Unmodification

Molecular Weight

~ 108 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of CYLD protein.

Applications Notes

Western blot analysis of CYLD expression in Hela (A) whole cell lysates.

Host or Source

Mouse

Available Sizes

Frequently Asked Questions

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