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PRAS40 monoclonal antibody

Product Specifications

Background

Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt. One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 proteins. PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity. PRAS40 interacts with raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1. mTORC1 in turn phosphorylates PRAS40 at Ser183.

CAS Number

9007-83-4

Structure Composition

Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

PRAS40; Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate

Swiss Prot

Q96B36

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein of human PRAS40. The exact sequence is proprietary.

Conjugation

Unconjugated

Related Pathways

Autophagy Signaling, PI3K AKT signaling, T Cell Receptor Signaling, Translational Contral elF4 and p70 S6 Kinase, mTOR Signaling

Applications

WB

Dilution

WB (1/500 - 1/2000)

Purity

This antibody is purified through a protein G column.

Modification

Unmodification

Molecular Weight

~ 40 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of PRAS40 protein.

Applications Notes

Western blot analysis of PRAS40 expression in 293 (A), Hela (B), MCF7 (C), NIH3T3 (D) whole cell lysates.

Host or Source

Mouse

Available Sizes

Frequently Asked Questions

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