LIN28B monoclonal antibody
Product Specifications
Background
LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs. The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2. It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs. LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy. In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans. Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells.
Structure Composition
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Name Alternative
CSDD2; Protein lin-28 homolog B; Lin-28B
Swiss Prot
Q6ZN17
Reactivity
Human
Immunogen
Recombinant fusion protein of human LIN28B. The exact sequence is proprietary.
Conjugation
Unconjugated
Applications
WB
Dilution
WB (1/1000 - 1/2000)
Purity
This antibody is purified through a protein G column.
Modification
Unmodification
Molecular Weight
~ 35 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of LIN28B protein.
Applications Notes
Western blot analysis of LIN28B expression in 293 (A), K562 (B), HepG2 (C) whole cell lysates.
Host or Source
Mouse
CAS Number
9007-83-4
Available Sizes
Curated Selection
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