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ULK3 monoclonal antibody

Product Specifications

Background

Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3) . Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.

CAS Number

9007-83-4

Structure Composition

Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

Serine/threonine-protein kinase ULK3; Unc-51-like kinase 3

Swiss Prot

Q6PHR2

Reactivity

Human, Mouse, Rat

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ULK3. The exact sequence is proprietary.

Conjugation

Unconjugated

Applications

WB

Dilution

WB (1/500 - 1/1000), IHC (1/50 - 1/200)

Purity

This antibody is purified through a protein G column.

Modification

Unmodification

Molecular Weight

~ 55 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of ULK3 protein.

Applications Notes

Western blot analysis of ULK3 expression in Hela (A), LNCaP (B), mouse testis (C), rat testis (D) whole cell lysates.

Host or Source

Mouse

Available Sizes

Frequently Asked Questions

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