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BAD (Phospho-S155) monoclonal antibody

Product Specifications

Background

Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL. Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136. Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xL. Akt phosphorylates Bad at Ser136 to promote cell survival. Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK and mitochondria-anchored PKA. Phosphorylation at Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL.

CAS Number

9007-83-4

Structure Composition

Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

BBC6; BCL2L8; Bcl2 antagonist of cell death; BAD; Bcl-2-binding component 6; Bcl-2-like protein 8; Bcl2-L-8; Bcl-XL/Bcl-2-associated death promoter

Swiss Prot

Q92934

Reactivity

Human, Mouse

Immunogen

KLH-conjugated synthetic peptide of human BAD (pS155) . The exact sequence is proprietary.

Conjugation

Unconjugated

Related Pathways

Regulation of Apoptosis, ErbB HER Signaling, MAPK Erk in Growth and Differentiation Pathway (, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis, PI3K AKT signaling

Applications

WB

Dilution

WB (1/500 - 1/1000)

Purity

This antibody is purified through a protein G column.

Modification

Phosphorylation

Molecular Weight

~ 23 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of BAD (pS155) protein.

Applications Notes

Western blot analysis of BAD (pS155) expression in 293 transfected with FlagBad (A) whole cell lysates.

Host or Source

Mouse

Available Sizes

Frequently Asked Questions

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