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CaMK2 beta monoclonal antibody

Product Specifications

Background

CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T cell receptor signaling. CaMKII has catalytic and regulatory domains. Ca2+/calmodulin binding to the CaMKII regulatory domain relieves autoinhibition and activates the kinase (3) . The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active. The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents phospho-CaMKII (Thr286) dephosphorylation through an inhibitory effect on PP1.

CAS Number

9007-83-4

Structure Composition

Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

CAM2; CAMK2; CAMKB; Calcium/calmodulin-dependent protein kinase type II subunit beta; CaM kinase II subunit beta; CaMK-II subunit beta

Swiss Prot

Q13554

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein of human CaMK2 beta. The exact sequence is proprietary.

Conjugation

Unconjugated

Related Pathways

ErbB HER Signaling, G Protein-coupled Receptors Signaling to MAPK Erk, Regulation of Microtubule Dynamics, Mitochondrial Control of Apoptosis

Applications

WB, IHC

Dilution

WB (1/500 - 1/1000), IHC (1/50 - 1/200)

Purity

This antibody is purified through a protein G column.

Modification

Unmodification

Molecular Weight

~ 60 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of CaMK2 beta protein.

Applications Notes

Western blot analysis of CaMK2 beta expression in Jurkat (A), PC12 (B), human brain (C), mouse heart (D) whole cell lysates.

Host or Source

Mouse

Available Sizes

Frequently Asked Questions

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