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PPAR alpha monoclonal antibody

Product Specifications

Background

Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC) . Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.

CAS Number

9007-83-4

Structure Composition

Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

NR1C1; PPAR; Peroxisome proliferator-activated receptor alpha; PPAR-alpha; Nuclear receptor subfamily 1 group C member 1

Swiss Prot

Q07869

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein of human PPAR alpha. The exact sequence is proprietary.

Conjugation

Unconjugated

Applications

WB, IHC, IF/ICC, FC

Dilution

WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/10 - 1/50), FC (1/10 - 1/50)

Purity

This antibody is purified through a protein G column.

Modification

Unmodification

Molecular Weight

~ 55 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of PPAR alpha protein.

Applications Notes

Western blot analysis of PPAR alpha expression in Hela (A), Jurkat (B), NIH3T3 (C) whole cell lysates.

Host or Source

Mouse

Available Sizes

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