14-3-3 zeta monoclonal antibody

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways . 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively . Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules . The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation independent interactions has been observed . 14-3-3 binding masks specific sequences of the target protein, and therefore, modulates target protein localization, phosphorylation state, stability, and molecular interactions . 14-3-3 proteins may also induce target protein conformational changes that modify target protein function . Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities . Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes .

Regulation of Actin Dynamics, Regulation of Apoptosis, Cell Cycle G2 M DNA Damage Signaling Pathway, MAPK Erk in Growth and Differentiation Pathway, hippo Signaling, Mitochondrial Control of Apoptosis, PI3K AKT signaling, SAPK JNK Signaling Cascades.