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Rbx1 monoclonal antibody

Product Specifications

Background

RING-box protein 1 (RBX1 or ROC1) is an essential component of two distinct but structurally related E3 ubiquitin ligase complexes, the SCF complex and the CBC (VHL) complex . RBX1 mediates the neddylation of CUL1, which activates SCF E3 ligase by facilitating the ubiquitin transfer from E2 to substrates . The RING finger domain of RBX1 is required for ubiquitin ligation . Two evolutionarily conserved mammalian RBX family members, RBX1/ROC1 and RBX2/ROC2/SAG, have been identified . RBX1 is constitutively expressed and binds to CUL2/VHL, while stress-inducible RBX2 binds to CUL5/SOCS .

CAS Number

9007-83-4

Structure Composition

Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

RNF75; ROC1; E3 ubiquitin-protein ligase RBX1; Protein ZYP; RING finger protein 75; RING-box protein 1; Rbx1; Regulator of cullins 1

Swiss Prot

P62877

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein of human Rbx1. The exact sequence is proprietary.

Conjugation

Unconjugated

Related Pathways

Tumor Angiogenesis.

Applications

WB

Dilution

WB (1/500 - 1/1000)

Purity

This antibody is purified through a protein G column.

Modification

Unmodification

Molecular Weight

~ 13 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of Rbx1 protein.

Applications Notes

Western blot analysis of Rbx1 expression in Hela (A), NIH3T3 (B) whole cell lysates.

Host or Source

Mouse

Available Sizes

Curated Selection

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