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RPS6 monoclonal antibody

Product Specifications

Background

One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation . Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions . These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation . Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of S6 protein .

CAS Number

9007-83-4

Structure Composition

Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

40S ribosomal protein S6; Phosphoprotein NP33

Swiss Prot

P62753

Reactivity

Human, Rat

Immunogen

Recombinant fusion protein of human RPS6. The exact sequence is proprietary.

Conjugation

Unconjugated

Related Pathways

MAPK Erk in Growth and Differentiation Pathway, PI3K AKT signaling, Translational Contral elF4 and p70 S6 Kinase.

Applications

WB, IHC, IF/ICC, FC

Dilution

WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/10 - 1/50), FC (1/10 - 1/50)

Purity

This antibody is purified through a protein G column.

Modification

Unmodification

Molecular Weight

~ 32 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of RPS6 protein.

Applications Notes

Western blot analysis of RPS6 expression in 293 (A), Hela (B), NIH3T3 (C), PC12 (D), rat brain (E) whole cell lysates.

Host or Source

Mouse

Available Sizes

Curated Selection

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