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BID monoclonal antibody

Product Specifications

Background

Bid is a pro-apoptotic “BH3 domain-only” member of the Bcl-2 family originally discovered to interact with both the anti-apoptotic family member Bcl-2 and the pro-apoptotic protein Bax . Bid is normally localized in the cytosolic fraction of cells as an inactive precursor and is cleaved at Asp60 by caspase-8 during Fas signaling, leading to translocation of the carboxyl terminal p15 fragment (tBid) to the mitochondrial outer membrane . Translocation of Bid is associated with release of cytochrome c from the mitochondria, leading to complex formation with Apaf-1 and caspase-9 and resulting in caspase-9 activation . Thus, Bid relays an apoptotic signal from the cell surface to the mitochondria triggering caspase activation .

CAS Number

9007-83-4

Structure Composition

Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

BH3-interacting domain death agonist; p22 BID; BID

Swiss Prot

P55957

Reactivity

Human

Immunogen

Recombinant fusion protein of human BID. The exact sequence is proprietary.

Conjugation

Unconjugated

Related Pathways

Regulation of Apoptosis, Death Receptor Signaling, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis.

Applications

WB, IHC, IF/ICC

Dilution

WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/10 - 1/50)

Purity

This antibody is purified through a protein G column.

Modification

Unmodification

Molecular Weight

~ 22 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of BID protein.

Applications Notes

Western blot analysis of BID expression in HeLa (A), A431 (B), MDAMB231 (C), Raji (D), Jurkat (E), THP1 (F) whole cell lysates.

Host or Source

Mouse

Available Sizes

Frequently Asked Questions

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