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LIMK1 monoclonal antibody

Product Specifications

Background

LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains . LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs, and ROCK . PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr508 or Thr505 residues in the activation loop, increasing LIMK activity . Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser3 residue of cofilin .

CAS Number

9007-83-4

Structure Composition

Mouse IgM kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

LIMK; LIM domain kinase 1; LIMK-1

Swiss Prot

P53667

Reactivity

Human, Mouse

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human LIMK1. The exact sequence is proprietary.

Conjugation

Unconjugated

Related Pathways

Regulation of Actin Dynamics, Regulation of Microtubule Dynamics, TGFβ Smad signaling.

Applications

WB

Dilution

WB (1/500 - 1/1000)

Purity

This antibody is purified through a protein G column.

Modification

Unmodification

Molecular Weight

~ 70 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of LIMK1 protein.

Applications Notes

Western blot analysis of LIMK1 expression in human brain (A), mouse brain (B) whole cell lysates.

Host or Source

Mouse

Available Sizes

Frequently Asked Questions

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