RAB27 monoclonal antibody
Product Specifications
Background
Rab27 is a member of the Ras superfamily of small Rab GTPases implicated in exocytosis . The protein is localized in secretory lysosomes, such as melanosomes in melanocyte or lytic granules in cytotoxic T cells to control exosome secretion pathway. Rab27 has two isoforms, Rab27a and Rab27b. Rab27a colocalizes with part of CD63 staining vesicles, and Rab27b shows perinuclear distribution. Target knock out studies indicate that the isoforms control different steps of the exosome secretion pathway . Rab27a interacts with a wide range of effectors and is involved in multiple steps of exocytosis depending on the effector it associated with and the cell type that is involved . Rab27a has been shown to be an important player in leukocyte function, cancer metastasis and invasion, and insulin secretion.
CAS Number
9007-83-4
Structure Composition
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Name Alternative
RAB27; Ras-related protein Rab-27A; Rab-27; GTP-binding protein Ram
Swiss Prot
P51159
Reactivity
Human
Immunogen
Recombinant fusion protein of human RAB27. The exact sequence is proprietary.
Conjugation
Unconjugated
Applications
WB, FC
Dilution
WB (1/500 - 1/1000), FC (1/10 - 1/50)
Purity
This antibody is purified through a protein G column.
Modification
Unmodification
Molecular Weight
~ 27 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of RAB27 protein.
Applications Notes
Western blot analysis of RAB27 expression in K562 (A) whole cell lysates.
Host or Source
Mouse
Available Sizes
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
