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P27 Kip1 monoclonal antibody

Product Specifications

Background

P27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin-2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway .

CAS Number

9007-83-4

Structure Composition

Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1

Swiss Prot

P46527

Reactivity

Human

Immunogen

Recombinant fusion protein of human p27 Kip1. The exact sequence is proprietary.

Conjugation

Unconjugated

Related Pathways

ErbB HER Signaling.

Applications

WB, IHC

Dilution

WB (1/500 - 1/1000), IHC (1/50 - 1/200)

Purity

This antibody is purified through a protein G column.

Modification

Unmodification

Molecular Weight

~ 27 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of p27 Kip1 protein.

Applications Notes

Western blot analysis of p27 Kip1 expression in MCF7 (A), U266 (B) whole cell lysates.

Host or Source

Mouse

Available Sizes

Curated Selection

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