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ZAP70 monoclonal antibody

Product Specifications

Background

The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement . Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck . Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia .

CAS Number

9007-83-4

Structure Composition

Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

SRK; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase

Swiss Prot

P43403

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein of human ZAP70. The exact sequence is proprietary.

Conjugation

Unconjugated

Related Pathways

T cell receptor signaling.

Applications

WB, IF/ICC, FC

Dilution

WB (1/500 - 1/1000), IF/ICC (1/10 - 1/50), FC (1/10 - 1/50)

Purity

This antibody is purified through a protein G column.

Modification

Unmodification

Molecular Weight

~ 70 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of ZAP70 protein.

Applications Notes

Western blot analysis of ZAP70 expression in Jurkat (A), MOLT4 (B), mouse thymus (C) whole cell lysates.

Host or Source

Mouse

Available Sizes

Curated Selection

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