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CDC25C monoclonal antibody

Product Specifications

Background

Cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis . cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint . When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm and thereby preventing premature mitosis . The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage .

CAS Number

9007-83-4

Structure Composition

Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

M-phase inducer phosphatase 3; Dual specificity phosphatase Cdc25C

Swiss Prot

P30307

Reactivity

Human

Immunogen

Recombinant fusion protein of human CDC25C. The exact sequence is proprietary.

Conjugation

Unconjugated

Related Pathways

Cell Cycle G2 M DNA Damage Signaling Pathway, G Protein-coupled Receptors Signaling to MAPK Erk, MAPK Erk in Growth and Differentiation Pathway.

Applications

WB, IHC

Dilution

WB (1/500 - 1/1000), IHC (1/50 - 1/200)

Purity

This antibody is purified through a protein G column.

Modification

Unmodification

Molecular Weight

~ 60 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of CDC25C protein.

Applications Notes

Western blot analysis of CDC25C expression in HepG2 (A), K562 (B), MCF7 (C), Raji (D) whole cell lysates.

Host or Source

Mouse

Available Sizes

Frequently Asked Questions

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