Calreticulin monoclonal antibody
Product Specifications
Background
Calcium is a universal signaling molecule involved in many cellular functions such as cell motility, metabolism, protein modification, protein folding, and apoptosis. Calcium is stored in the endoplasmic reticulum (ER), where it is buffered by calcium binding chaperones such as calnexin and calreticulin, and is released via the IP3 Receptor channel . Calreticulin also functions as an ER chaperone that ensures proper folding and quality control of newly synthesized glycoproteins. As such, calreticulin presumably does not alter protein folding but regulates proper timing for efficient folding and subunit assembly. Furthermore, calreticulin retains proteins in non-native conformation within the ER and targets them for degradation .
CAS Number
9007-83-4
Structure Composition
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Name Alternative
CRTC; Calreticulin; CRP55; Calregulin; Endoplasmic reticulum resident protein 60; ERp60; HACBP; grp60
Swiss Prot
P27797
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein of human Calreticulin. The exact sequence is proprietary.
Conjugation
Unconjugated
Applications
WB
Dilution
WB (1/500 - 1/1000)
Purity
This antibody is purified through a protein G column.
Modification
Unmodification
Molecular Weight
~ 60 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of Calreticulin protein.
Applications Notes
Western blot analysis of Calreticulin expression in HepG2 (A), Jurkat (B), HeLa (C), MCF7 (D), SHSY5Y (E), K562 (F), NIH3T3 (G), C6 (H), CHO (I) whole cell lysates.
Host or Source
Mouse
Available Sizes
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