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Filamin A monoclonal antibody

Product Specifications

Background

Filamins are a family of dimeric actin binding proteins that function as structural components of cell adhesion sites. They also serve as a scaffold for subcellular targeting of signaling molecules. The actin binding domain located at the amino terminus is followed by as many as 24 tandem repeats of about 96 residues and the dimerization domain is located at the carboxy terminus. In addition to actin filaments, filamins associate with other structural and signaling molecules such as -integrins, Rho/Rac/Cdc42, PKC and the insulin receptor, primarily through the carboxy-terminal dimerization domain . Filamin A, the most abundant, and filamin B are widely expressed isoforms, while filamin C is predominantly expressed in muscle. Filamin A is phosphorylated by PAK1 at Ser2152, which is required for PAK1-mediated actin cytoskeleton reorganization.

CAS Number

9007-83-4

Structure Composition

Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

FLN; FLN1; Filamin-A; FLN-A; Actin-binding protein 280; ABP-280; Alpha-filamin; Endothelial actin-binding protein; Filamin-1; Non-muscle filamin

Swiss Prot

P21333

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein of human Filamin A. The exact sequence is proprietary.

Conjugation

Unconjugated

Related Pathways

Regulation of Actin Dynamics, MAPK Erk in Growth and Differentiation Pathway

Applications

WB, IF/ICC

Dilution

WB (1/1000 - 1/2000), IF/ICC (1/10 - 1/50)

Purity

This antibody is purified through a protein G column.

Modification

Unmodification

Molecular Weight

~ 280 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of Filamin A protein.

Applications Notes

Western blot analysis of Filamin A expression in Hela (A), C6 (B), PC3 (C), COS7 (D), NIH3T3 (E) whole cell lysates.

Host or Source

Mouse

Available Sizes

Frequently Asked Questions

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