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MGMT monoclonal antibody

Product Specifications

Background

MGMT is a DNA repair enzyme that participates in a suicide reaction that specifically removes methyl or alkyl groups from the O position of guanine, restoring guanine to its normal form without causing DNA breaks . MGMT protects cells from alkylating toxins, and is an important factor in drug resistance to alkylating therapeutic agents . It is ubiquitously expressed in normal human tissues and is overexpressed in many types of human tumors, but epigenetically silenced in other tumors. MGMT silencing is a marker associated with poor prognosis, but is a good predictive marker for response to alkylating agent chemotherapy .

CAS Number

9007-83-4

Structure Composition

Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

Methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase

Swiss Prot

P16455

Reactivity

Human

Immunogen

Recombinant fusion protein of human MGMT. The exact sequence is proprietary.

Conjugation

Unconjugated

Applications

WB, FC

Dilution

WB (1/500 - 1/1000), IF/ICC (1/10 - 1/50)

Purity

This antibody is purified through a protein G column.

Modification

Unmodification

Molecular Weight

~ 22 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of MGMT protein.

Applications Notes

Western blot analysis of MGMT expression in Jurkat (A), MCF7 (B), MOLT4 (C), Raji (D) whole cell lysates.

Host or Source

Mouse

Available Sizes

Frequently Asked Questions

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