MUC1 monoclonal antibody
Product Specifications
Background
Mucins represent a family of glycoproteins characterized by repeat domains and dense O-glycosylation . MUC1 is aberrantly overexpressed in most human carcinomas. Increased expression of MUC1 in carcinomas reduces cell-cell and cell-ECM interactions. MUC1 is cleaved proteolytically, and the large ectodomain can remain associated with the small 25 kDa carboxy-terminal domain that contains a transmembrane segment and a 72-residue cytoplasmic tail . MUC1 interacts with ErbB family receptors and potentiates ERK1/2 activation . MUC1 also interacts with -catenin, which is regulated by GSK-3, PKC, and Src through phosphorylation at Ser44, Thr41, and Tyr46 of the MUC1 cytoplasmic tail . Overexpression of MUC1 potentiates transformation and attenuates stress-induced apoptosis through the Akt or p53 pathways .
CAS Number
9007-83-4
Structure Composition
Mouse IgG2b. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Name Alternative
PUM; Mucin-1; MUC-1; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut-reactive urinary mucin; PUM; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial membrane antigen; EMA; Tumor-associated mucin; CD227
Swiss Prot
P15941
Reactivity
Human
Immunogen
Recombinant fusion protein of human MUC1. The exact sequence is proprietary.
Conjugation
Unconjugated
Related Pathways
ErbB HER Signaling
Applications
WB
Dilution
WB (1/500 - 1/1000)
Purity
This antibody is purified through a protein G column.
Modification
Unmodification
Molecular Weight
~ 27 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of MUC1 protein.
Applications Notes
Western blot analysis of MUC1 expression in MDAMB435 (A) whole cell lysates.
Host or Source
Mouse
Available Sizes
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