C-SRC monoclonal antibody
Product Specifications
Background
The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells . Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active .
CAS Number
9007-83-4
Structure Composition
Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Name Alternative
SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src
Swiss Prot
P12931
Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein of human c-SRC. The exact sequence is proprietary.
Conjugation
Unconjugated
Related Pathways
Regulation of Actin Dynamics, Adherens Junction signaling, Tumor Angiogenesis, ErbB HER Signaling, G Protein-coupled Receptors Signaling to MAPK Erk, Jak Stat : IL-6 Receptor Signaling, Inhibition of Apoptosis, Regulation of Microtubule Dynamics, Signaling Pathways Activating p38 MAP Kinase, Wntβ catenin signaling
Applications
WB, IF/ICC
Dilution
WB (1/500 - 1/1000), IF/ICC (1/10 - 1/50)
Purity
This antibody is purified through a protein G column.
Modification
Unmodification
Molecular Weight
~ 60 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of c-SRC protein.
Applications Notes
Western blot analysis of c-SRC expression in HT29 (A) whole cell lysates.
Host or Source
Mouse
Available Sizes
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