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SPARC monoclonal antibody

Product Specifications

Background

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.

CAS Number

9007-83-4

Structure Composition

Mouse IgG1. Supplied in crude ascites with 0.01% sodium azide.

Product Name Alternative

ON; SPARC; Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine

Swiss Prot

P09486

Reactivity

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SPARC. The exact sequence is proprietary.

Conjugation

Unconjugated

Applications

WB

Dilution

WB (1/500 - 1/1000)

Modification

Unmodification

Molecular Weight

~ 40 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of SPARC protein.

Applications Notes

Western blot analysis of SPARC expression in Hela (A) whole cell lysates.

Host or Source

Mouse

Available Sizes

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