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CHRM2 monoclonal antibody

Product Specifications

Background

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate ; this then triggers calcium ion release into the cytosol.

CAS Number

9007-83-4

Structure Composition

Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

Muscarinic acetylcholine receptor M2

Swiss Prot

P08172

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein of human CHRM2. The exact sequence is proprietary.

Conjugation

Unconjugated

Applications

WB, IHC, IF/ICC, FC

Dilution

WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/10 - 1/50), FC (1/10 - 1/50)

Purity

This antibody is purified through a protein G column.

Modification

Unmodification

Molecular Weight

~ 55 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of CHRM2 protein.

Applications Notes

Western blot analysis of CHRM2 expression in SHSY5Y (A), human brain (B), mouse brain (C) whole cell lysates.

Host or Source

Mouse

Available Sizes

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