SP1 monoclonal antibody
Product Specifications
Background
Sp1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 was initially identified as a HeLa cell-derived factor that selectively activates in vitro transcription from the SV40 promoter and binds to the multiple GC boxes in the 21 bp repeated elements in SV40. The sequence specificity of DNA binding is conferred by Zn (II) fingers, whereas a different region of Sp1 appears to regulate the affinity of DNA binding. Sp1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences. Evidence suggests that the early growth response gene, Erg-1 (also known as Zif268 or NGF1-A), may downregulate certain mammalian gene promoters by competing with Sp1 for binding to an overlapping binding motif. The gene encoding human Sp1 maps to chromosome 12q13.13.
CAS Number
9007-83-4
Structure Composition
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Name Alternative
TSFP1; Transcription factor Sp1
Swiss Prot
P08047
Reactivity
Human
Immunogen
Recombinant fusion protein of human SP1. The exact sequence is proprietary.
Conjugation
Unconjugated
Applications
WB, IF/ICC, FC
Dilution
WB (1/500 - 1/1000), IF/ICC (1/10 - 1/50), FC (1/10 - 1/50)
Purity
This antibody is purified through a protein G column.
Modification
Unmodification
Molecular Weight
~ 105 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of SP1 protein.
Applications Notes
Western blot analysis of SP1 expression in Hela (A), Ramos (B) whole cell lysates.
Host or Source
Mouse
Available Sizes
Curated Selection
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