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Alpha-enolase monoclonal antibody

Product Specifications

Background

Enolases have been characterized as highly conserved cytoplasmic glycolytic enzymes that may be involved in differentiation. Three isoenzymes have been identified: a Enolase, b Enolase and g Enolase. a Enolase expression has been detected on most tissues, whereas b Enolase is expressed predominantly in muscle tissue and g enolase is detected only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway.

CAS Number

9007-83-4

Structure Composition

Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

ENO1L1; MBPB1; MPB1; Alpha-enolase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein

Swiss Prot

P06733

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein of human Alpha-enolase. The exact sequence is proprietary.

Conjugation

Unconjugated

Applications

WB, IHC

Dilution

WB (1/500 - 1/1000), IHC (1/50 - 1/200)

Purity

This antibody is purified through a protein G column.

Modification

Unmodification

Molecular Weight

~ 51 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of Alpha-enolase protein.

Applications Notes

Western blot analysis of Alpha-enolase expression in MCF7 (A), A431 (B), Hela (C), HepG2 (D), NIH3T3 (E), mouse stomach (F), mouse liver (G) whole cell lysates.

Host or Source

Mouse

Available Sizes

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