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ISG15 monoclonal antibody

Product Specifications

Background

Interferon-induced 15 kDa protein (ISG15) acts as ubiquitin by conjugating to intracellular target proteins such as JAK1 or MAPK3/ERK1 through an enzyme pathway distinct from that of ubiquitin. ISG15 shows specific chemotactic activity towards neutrophils and activates them to induce the release of eosinophil chemotactic factors. ISG15 is also involved in paracrine, autocrine and endocrine mechanisms by inducing IFN-g secretion by monocytes and macrophages, as in cell-to-cell signaling. ISG15 is a cytoplasmic protein expressed mainly in muscle, epithelial, neuronal and lymphoid cells.

CAS Number

9007-83-4

Structure Composition

Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

G1P2; UCRP; Ubiquitin-like protein ISG15; Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; IP17; Ubiquitin cross-reactive protein; hUCRP

Swiss Prot

P05161

Reactivity

Human

Immunogen

Recombinant fusion protein of human ISG15. The exact sequence is proprietary.

Conjugation

Unconjugated

Applications

WB, IF/ICC

Dilution

WB (1/500 - 1/4000), IF/ICC (1/10 - 1/50)

Purity

This antibody is purified through a protein G column.

Modification

Unmodification

Molecular Weight

~ 15 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of ISG15 protein.

Applications Notes

Western blot analysis of ISG15 expression in Hela (A), MCF7 (B) whole cell lysates.

Host or Source

Mouse

Available Sizes

Frequently Asked Questions

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