CNOT4 monoclonal antibody
Product Specifications
Background
The CCR4-NOT complex is an evolutionarily conserved, multi-component complex known to be involved in transcription as well as mRNA degradation. Various subunits within the complex are involved in influencing nuclear hormone receptor activities. The CCR4-NOT complex is also involved in the regulation of Histone H3 lysine 4 methylation through a ubiquitin-dependent pathway that likely involves the proteasome. CNOT4 (CCR4-NOT transcription complex subunit 4), also known as CCR4-associated factor 4 and E3 ubiquitin-protein ligase CNOT4, is a 575 amino acid protein that is a subunit of the CCR4-NOT complex. CNOT4 contains one C3H1-type zinc finger, one RING-type zinc finger and one RRM (RNA recognition motif) domain. Via its RING domain, CNOT4 binds E2 ubiquitin ligases. CNOT4 functions as a UbcH5B-dependent ubiquitin-protein ligase (E3 ligase) . There are eight isoforms of CNOT4 that are expressed as a result of alternative splicing events.
CAS Number
9007-83-4
Structure Composition
Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Name Alternative
NOT4; CCR4-NOT transcription complex subunit 4; CCR4-associated factor 4; E3 ubiquitin-protein ligase CNOT4; Potential transcriptional repressor NOT4Hp
Swiss Prot
O95628
Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein of human CNOT4. The exact sequence is proprietary.
Conjugation
Unconjugated
Applications
WB
Dilution
WB (1/500 - 1/4000)
Purity
This antibody is purified through a protein G column.
Modification
Unmodification
Molecular Weight
~ 63 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of CNOT4 protein.
Applications Notes
Western blot analysis of CNOT4 expression in 293 (A) whole cell lysates.
Host or Source
Mouse
Available Sizes
Curated Selection
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