Nephrin monoclonal antibody
Product Specifications
Background
Nephrin is a member of the immunoglobulin family of cell adhesion molecules that localizes to opposing sites of the secondary foot processes formed by podocytes, a specialized epithelial cell that ensures size- and charge-selective ultrafiltration. The human nephrin gene maps to chromosome 19q13.12 and encodes a 1,241 amino acid protein that contains a transmembrane domain, eight Ig-like modules, and one fibronectin III-like module. Nephrin is expressed in embryonic and adult kidneys and localizes to glomerular podocytes and the glomerular slit diaphragm. Nephrin stimulates mitogen-activated protein kinases and is enhanced by podocin, which binds to the cytoplasmic tail of nephrin. A293 cells treated with phorbol-12-myristate-13-acetate can upregulate nephrin, suggesting that protein kinase C is part of an intracellular signalling system, which regulates nephrin.
CAS Number
9007-83-4
Structure Composition
Mouse IgM kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Name Alternative
NPHN; Nephrin; Renal glomerulus-specific cell adhesion receptor
Swiss Prot
O60500
Reactivity
Human
Immunogen
Recombinant fusion protein of human Nephrin. The exact sequence is proprietary.
Conjugation
Unconjugated
Applications
WB
Dilution
WB (1/500 - 1/1000)
Purity
This antibody is purified through a protein G column.
Modification
Unmodification
Molecular Weight
~ 180 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of Nephrin protein.
Applications Notes
Western blot analysis of Nephrin expression in HepG2 (A) whole cell lysates.
Host or Source
Mouse
Available Sizes
Curated Selection
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