CYP27B1 monoclonal antibody
Product Specifications
Background
A cytochrome P450 monooxygenase involved in vitamin D metabolism and in calcium and phosphorus homeostasis. Catalyzes the rate-limiting step in the activation of vitamin D in the kidney, namely the hydroxylation of 25-hydroxyvitamin D3/calcidiol at the C1alpha-position to form the hormonally active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3/calcitriol that acts via the vitamin D receptor (VDR) . Has 1alpha-hydroxylase activity on vitamin D intermediates of the CYP24A1-mediated inactivation pathway. Converts 24R,25-dihydroxyvitamin D3/secalciferol to 1-alpha,24,25-trihydroxyvitamin D3, an active ligand of VDR. Also active on 25-hydroxyvitamin D2. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin.
CAS Number
9007-83-4
Structure Composition
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Name Alternative
CYP1ALPHA; CYP27B; 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial; 25-OHD-1 alpha-hydroxylase; 25-hydroxyvitamin D (3) 1-alpha-hydroxylase; VD3 1A hydroxylase; Calcidiol 1-monooxygenase; Cytochrome P450 subfamily XXVIIB polypeptide 1; Cytochrome P450C1 alpha; Cytochrome P450VD1-alpha; Cytochrome p450 27B1
Swiss Prot
O15528
Reactivity
Human
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CYP27B1. The exact sequence is proprietary.
Conjugation
Unconjugated
Applications
WB
Dilution
WB (1/500 - 1/1000)
Purity
This antibody is purified through a protein G column.
Modification
Unmodification
Molecular Weight
~ 55 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of CYP27B1 protein.
Applications Notes
Western blot analysis of CYP27B1 expression in MCF7 (A) whole cell lysates.
Host or Source
Mouse
Available Sizes
Curated Selection
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