RAB27B monoclonal antibody
Product Specifications
Background
Rab27 proteins are members of the Ras superfamily of small Rab GTPases associated with the process of exocytosis, and in particular the exosome secretion pathway. There are two Rab27 isoforms, Rab27A and Rab27B, which are encoded by separate genes and which exhibit distinct subcellular localization and functions. Rab27A colocalizes to CD63+ vesicles, whereas Rab27B is observed in perinuclear vesicles. Targeted knockdown studies suggest that the isoforms control distinct steps of the exosome secretion pathway. Rab27A participates in docking and membrane fusion from multivesicular endosomes (MVEs) to exosomes, whereas Rab27B mediates the transfer of membrane vesicles from the trans-Golgi network to MVEs. It has been suggested that Rab27A and Rab27B may have importance in exosome secretion by cancer cells.
CAS Number
9007-83-4
Structure Composition
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Name Alternative
Ras-related protein Rab-27B; C25KG
Swiss Prot
O00194
Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein of human RAB27B. The exact sequence is proprietary.
Conjugation
Unconjugated
Applications
WB, FC
Dilution
WB (1/500 - 1/1000), FC (1/10 - 1/50)
Purity
This antibody is purified through a protein G column.
Modification
Unmodification
Molecular Weight
~ 27 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of RAB27B protein.
Applications Notes
Western blot analysis of RAB27B expression in DU145 (A), mouse stomach (B) whole cell lysates.
Host or Source
Mouse
Available Sizes
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