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Gamma-enolase monoclonal antibody

Product Specifications

Background

Enolase is a glycolytic enzyme that is involved in the conversion of 2-phosphoglycerate to phosphoenolpyruvate. Mammalian enolase has three subunits: α, β, and γ, that can form homo and heterodimers. Homodimers of γ enolase are neuronal-specific. Research studies have shown elevated levels of neuro-specific enolase-2 in neuroblastoma and small-cell lung cancer.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE

Swiss Prot

P09104

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human NSE.

Conjugation

Unconjugated

Applications

WB, E

Dilution

WB (1/500 - 1/1000), E (1/5000 - 1/10000)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Unmodification

Molecular Weight

~ 47 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of Gamma-enolase protein.

Applications Notes

Western blot analysis of Gamma-enolase expression in SHSY5Y (A), U87MG (B), rat brain (C), mouse brain (D) whole cell lysates.

Host or Source

Mouse

Available Sizes

Frequently Asked Questions

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