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Lamin A/C monoclonal antibody

Product Specifications

Background

Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization. Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment. The cleavage of lamins results in nuclear dysregulation and cell death.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

LMN1; Prelamin-A/C

Swiss Prot

P02545

Reactivity

Human, Mouse, Rat

Immunogen

Purified recombinant human LMNA protein fragments expressed in E.coli.

Conjugation

Unconjugated

Related Pathways

Death Receptor Signaling, PI3K AKT signaling.

Applications

WB, IF/ICC

Dilution

WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Unmodification

Molecular Weight

~ 74, 63 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of Lamin A/C protein.

Applications Notes

Western blot analysis of Lamin A/C expression in MCF7 (A), A549 (B), Hela (C) whole cell lysates.

Host or Source

Mouse

Available Sizes

Frequently Asked Questions

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