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EGFR monoclonal antibody

Product Specifications

Background

The epidermal growth factor (EGF) receptor is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Research studies have shown that somatic mutations in the tyrosine kinase domain of EGF receptor (EGFR) are present in a subset of lung adenocarinomas that respond to EGFR inhibitors, such as gefinitib and erlotinib. Two types of mutations account for approximately 90% of mutated cases: a specific point mutation, L858R, that occurs in exon 21 and short in-frame deletions in exon 19. The most frequent exon 19 deletion is E746-A750, accounting for 90% of lesions at this site, although some rare variants occur.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Swiss Prot

P00533

Reactivity

Human, Monkey

Immunogen

Purified recombinant human EGFR protein fragments expressed in E.coli.

Conjugation

Unconjugated

Related Pathways

Tumor Angiogenesis, ErbB HER Signaling, Jak Stat : IL-6 Receptor Signaling, Phospholipase Signaling.

Applications

WB, IF/ICC, IP

Dilution

WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Unmodification

Molecular Weight

~ 175 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of EGFR protein.

Applications Notes

Western blot analysis of EGFR expression in A549 (A), MDAMB468 (B), COS7 (C) whole cell lysates.

Host or Source

Mouse

Available Sizes

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