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Hexokinase 1 monoclonal antibody

Product Specifications

Background

Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

Hexokinase-1; Brain form hexokinase; Hexokinase type I; HK I

Swiss Prot

P19367

Reactivity

Human, Mouse, Rat

Immunogen

Purified recombinant human Hexokinase 1 protein fragments expressed in E.coli

Conjugation

Unconjugated

Related Pathways

Warburg Effect

Applications

WB

Dilution

WB (1/500 - 1/1000)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Unmodification

Molecular Weight

~ 102 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of Hexokinase 1 protein.

Applications Notes

Western blot analysis of Hexokinase 1 expression in MCF7 (A), Jurkat (B), CHOK1 (C), C2C12 (D), C6 (E), NIH3T3 (F), Hela (G) whole cell lysates.

Host or Source

Mouse

Available Sizes

Frequently Asked Questions

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