GRP78 monoclonal antibody
Product Specifications
Background
Secretory and transmembrane proteins are synthesized on polysomes and translocated into the endoplasmic reticulum (ER) . Inside the ER, these proteins are often modified by disulfide bond formation, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including BiP. BiP was identified as an immunoglobulin heavy chain binding protein in pre-B cells. It was also found to be induced at the protein level by glucose starvation. When protein folding is disturbed inside ER, BiP synthesis is increased. Subsequently, BiP binds to misfolded proteins to prevent them from forming aggregates and assists in proper refolding.
CAS Number
9007-83-4
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Product Name Alternative
GRP78; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca (2+) -binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP
Swiss Prot
P11021
Reactivity
Human, Mouse, Rat
Immunogen
Purified recombinant human BiP/GRP78 protein expressed in E.coli.
Conjugation
Unconjugated
Applications
WB, IF/ICC, IP
Dilution
WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)
Purity
The antibody was purified by immunogen affinity chromatography.
Modification
Unmodification
Molecular Weight
~ 78 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of GRP78 protein.
Applications Notes
Western blot analysis of GRP78 expression in NIH3T3 (A), A549 (B), C6 (C), Hela (D) whole cell lysates.
Host or Source
Mouse
Available Sizes
Curated Selection
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