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GAPDH monoclonal antibody

Product Specifications

Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis, gene expression, and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

GAPD; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH

Swiss Prot

P04406

Reactivity

Human, Mouse, Rat, Hamster, Monkey, Yeast

Immunogen

Purified recombinant human GAPDH protein fragments expressed in E.coli.

Conjugation

Unconjugated

Applications

WB

Dilution

WB (1/500 - 1/1000)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Unmodification

Molecular Weight

~ 37 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of GAPDH protein.

Applications Notes

Western blot analysis of GAPDH expression in Hela (A), NIH3T3 (B), C6 (C), COS7 (D), CHOK1 (E), rat brain (F), Jurkat (G) whole cell lysates.

Host or Source

Mouse

Available Sizes

Frequently Asked Questions

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