MLH1 monoclonal antibody
Product Specifications
Background
Mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. MLH1 is the human homologue of the E. coli MMR gene mutL. MMR requires recognition of a base mismatch or insertion/deletion loop by a MutS homolog followed by recruitment of a MutL heterodimeric complex consisting of MLH1 and PMS1 (MutL-γ), PMS2 (MutL-α), or MLH3 (MutL-γ) . Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases, and DNA ligase. Inactivation of the MLH1 gene causes genome instability and predisposition to cancer. The MLH1 gene is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC) . MLH1 also plays a role in meiotic recombination.
CAS Number
9007-83-4
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Product Name Alternative
COCA2; DNA mismatch repair protein Mlh1; MutL protein homolog 1
Swiss Prot
P40692
Reactivity
Human, Monkey
Immunogen
Purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli.
Conjugation
Unconjugated
Applications
WB, IF/ICC
Dilution
WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100)
Purity
The antibody was purified by immunogen affinity chromatography.
Modification
Unmodification
Molecular Weight
~ 85 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of MLH1 protein.
Applications Notes
Western blot analysis of MLH1 expression in Hela (A), Jurkat (B), 293T (C), SW480 (D) whole cell lysates.
Host or Source
Mouse
Available Sizes
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