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HARS monoclonal antibody

Product Specifications

Background

Catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP) . Plays a role in axon guidance.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

HRS; Histidine--tRNA ligase cytoplasmic; Histidyl-tRNA synthetase; HisRS

Swiss Prot

P12081

Reactivity

Human, Mouse, Monkey

Immunogen

Purified recombinant human HARS protein fragments expressed in E.coli.

Conjugation

Unconjugated

Applications

WB

Dilution

WB (1/500 - 1/1000)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Unmodification

Molecular Weight

~ 55 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of HARS protein.

Applications Notes

Western blot analysis of HARS expression in T47D (A), NIH3T3 (B), COS7 (C), C6 (D), Hela (E) whole cell lysates.

Host or Source

Mouse

Available Sizes

Curated Selection

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