MKK6 monoclonal antibody
Product Specifications
Background
MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase. MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site, Thr-Gly-Tyr, but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines. Activation of MKK3 and MKK6 occurs through phosphorylation at Ser189 and Thr222 on MKK3 and Ser207 and Thr211 on MKK6.
CAS Number
9007-83-4
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Product Name Alternative
MEK6; MKK6; PRKMK6; SKK3; Dual specificity mitogen-activated protein kinase kinase 6; MAP kinase kinase 6; MAPKK 6; MAPK/ERK kinase 6; MEK 6; Stress-activated protein kinase kinase 3; SAPK kinase 3; SAPKK-3; SAPKK3
Swiss Prot
P52564
Reactivity
Human, Rat
Immunogen
Purified recombinant human MKK6 protein fragments expressed in E.coli.
Conjugation
Unconjugated
Related Pathways
Regulation of Actin Dynamics, ErbB HER Signaling, G Protein-coupled Receptors Signaling to MAPK Erk, Jak Stat : IL-6 Receptor Signaling, TGFβ Smad signaling
Applications
WB, IP
Dilution
WB (1/500 - 1/1000), IP (1/10 - 1/50)
Purity
The antibody was purified by immunogen affinity chromatography.
Modification
Unmodification
Molecular Weight
~ 38 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of MKK6 protein.
Applications Notes
Western blot analysis of MKK6 expression in PC12 (A), C6 (B), Ramos (C), Jurkat (D), Hela (E) whole cell lysates.
Host or Source
Mouse
Available Sizes
Curated Selection
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