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STAT5A monoclonal antibody

Product Specifications

Background

Stat5 is activated in response to a wide variety of ligands, including IL-2, GM-CSF, growth hormone, and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation. This phosphorylation is mediated by Src upon erythropoietin stimulation. Stat5 is constitutively active in some leukemic cell types. Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility. Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

STAT5; Signal transducer and activator of transcription 5A

Swiss Prot

P42229

Reactivity

Human, Mouse

Immunogen

Purified recombinant human Stat5a protein fragments expressed in E.coli.

Conjugation

Unconjugated

Related Pathways

ErbB HER Signaling, Jak Stat : IL-6 Receptor Signaling

Applications

WB

Dilution

WB (1/500 - 1/1000)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Unmodification

Molecular Weight

~ 90 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of STAT5A protein.

Applications Notes

Western blot analysis of STAT5A expression in NIH3T3 (A), A549 (B), Molt4 (C), K562 (D), Hela (E) whole cell lysates.

Host or Source

Mouse

Available Sizes

Curated Selection

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