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FEN1 monoclonal antibody

Product Specifications

Background

Flap endonuclease-1 (FEN-1) is a structure-specific nuclease with multiple functions in DNA processing pathways. The replication and DNA repair activities of FEN-1 are critical for genomic stability in the eukaryotic cell. Through interaction with proliferation cell nuclear antigen (PCNA), FEN-1 helps coordinate Okazaki fragment maturation by removing RNA-DNA primers. FEN-1 is also required for non-homologous end joining of double-stranded DNA breaks in long patch base excision repair. The multi-functional activities of FEN-1 are regulated by various mechanisms, including protein partner interactions, post-translational modifications, and subcellular re-localization in response to cell cycle or DNA damage.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

RAD2; Flap endonuclease 1; FEN-1; DNase IV; Flap structure-specific endonuclease 1; Maturation factor 1; MF1; hFEN-1

Swiss Prot

P39748

Reactivity

Human, Mouse, Rat, Monkey, Hamster

Immunogen

Purified recombinant human FEN-1 protein fragments expressed in E.coli.

Conjugation

Unconjugated

Applications

WB, IF/ICC

Dilution

WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Unmodification

Molecular Weight

~ 45 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of FEN1 protein.

Applications Notes

Western blot analysis of FEN1 expression in Hela (A), Jurkat (B), NIH3T3 (C), COS7 (D), PC12 (E), C6 (F), Raw264.7 (G) whole cell lysates.

Host or Source

Mouse

Available Sizes

Frequently Asked Questions

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