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STAT1 monoclonal antibody

Product Specifications

Background

The Stat1 transcription factor is activated in response to a large number of ligands and is essential for responsiveness to IFN-α and IFN-γ. Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding. Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa) . In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors. In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK) -dependent pathway in response to IFN-α and other cellular stresses. Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84

Swiss Prot

P42224

Reactivity

Human, Monkey

Immunogen

Purified recombinant human STAT1 protein fragments expressed in E.coli

Conjugation

Unconjugated

Related Pathways

ErbB HER Signaling, Jak Stat : IL-6 Receptor Signaling, Inhibition of Apoptosis

Applications

WB, IP

Dilution

WB (1/500 - 1/1000), IP (1/10 - 1/50)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Unmodification

Molecular Weight

~ 84 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of STAT1 protein.

Applications Notes

Western blot analysis of STAT1 expression in Hela (A), MCF7 (B), COS7 (C), SW480 (D), A549 (E) whole cell lysates.

Host or Source

Mouse

Available Sizes

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