STAT3 monoclonal antibody

Product Specifications

Background

The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors and is required for murine fetal development. Research studies have shown that Stat3 is constitutively activated in a number of human tumors and possesses oncogenic potential and anti-apoptotic activities. Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding. Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways. Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage. It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

APRF; Signal transducer and activator of transcription 3; Acute-phase response factor

Swiss Prot

P40763

Reactivity

Human, Mouse, Rat, Monkey, Hamster

Immunogen

Purified recombinant human STAT3 protein fragments expressed in E.coli

Conjugation

Unconjugated

Related Pathways

ErbB HER Signaling, Jak Stat : IL-6 Receptor Signaling, Inhibition of Apoptosis, Regulation of Microtubule Dynamics, SAPK JNK Signaling Cascades

Applications

Dilution

WB (1/500 - 1/1000)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Unmodification

Molecular Weight

~ 79 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of STAT3 protein.

Applications Notes

Western blot analysis of STAT3 expression in C6 (A), NIH3T3 (B), K562 (C), Jurkat (D), Hela (E), MCF7 (F), COS7 (G) whole cell lysates.

Host or Source

Mouse

Available Sizes

Curated Selection

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